Transcription factors with Perturb-seq knockdown data for C19orf81. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = C19orf81 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of C19orf81, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr19:50,368,630–50,369,092 | 289.6 kb | Distal (>10kb) Multiome | 311 | |
| chr19:50,376,225–50,377,452 | 281.9 kb | Distal (>10kb) Multiome | 931 | |
| chr19:50,383,444–50,384,805 | 274.3 kb | Distal (>10kb) Multiome | 863 | |
| chr19:50,414,638–50,415,204 | 243.6 kb | Distal (>10kb) Multiome | 377 | |
| chr19:50,431,430–50,432,503 | 226.7 kb | Distal (>10kb) Multiome | 530 | |
| chr19:50,475,914–50,477,044 | 181.9 kb | Distal (>10kb) Multiome | 622 | |
| chr19:50,510,707–50,511,948 | 147.1 kb | Distal (>10kb) Multiome | 794 | |
| chr19:50,556,266–50,557,084 | 101.8 kb | Distal (>10kb) Multiome | 463 | |
| chr19:50,639,246–50,640,210 | 18.8 kb | Distal (>10kb) Multiome | 326 | |
| chr19:50,649,410–50,649,829 | 8.7 kb | Proximal (<10kb) | 228 | |
| chr19:50,657,814–50,659,557 | 217 bp | At TSS Multiome | 790 | |
| chr19:50,723,765–50,725,772 | 66.4 kb | Distal (>10kb) Multiome | 721 | |
| chr19:50,728,202–50,728,738 | 70.0 kb | Distal (>10kb) Multiome | 345 | |
| chr19:50,804,189–50,805,375 | 146.4 kb | Distal (>10kb) Multiome | 919 | |
| chr19:50,835,543–50,836,598 | 177.6 kb | Distal (>10kb) Multiome | 561 | |
| chr19:50,909,047–50,910,046 | 251.1 kb | Distal (>10kb) Multiome | 239 | |
| chr19:50,921,645–50,922,754 | 263.7 kb | Distal (>10kb) Multiome | 450 |
Genomic view of the C19orf81 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.