Transcription factors with Perturb-seq knockdown data for C19orf25. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = C19orf25 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of C19orf25, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr19:1,194,370–1,194,985 | 284.6 kb | Distal (>10kb) Multiome | 531 | |
| chr19:1,205,175–1,206,374 | 273.6 kb | Distal (>10kb) Multiome | 746 | |
| chr19:1,237,080–1,238,434 | 241.1 kb | Distal (>10kb) Multiome | 430 | |
| chr19:1,239,513–1,242,539 | 237.5 kb | Distal (>10kb) Multiome | 889 | |
| chr19:1,244,253–1,244,857 | 234.6 kb | Distal (>10kb) Multiome | 590 | |
| chr19:1,247,981–1,252,501 | 228.1 kb | Distal (>10kb) Multiome | 1136 | |
| chr19:1,259,185–1,262,955 | 219.8 kb | Distal (>10kb) Multiome | 1070 | |
| chr19:1,265,280–1,270,235 | 209.9 kb | Distal (>10kb) Multiome | 1171 | |
| chr19:1,274,423–1,276,502 | 203.5 kb | Distal (>10kb) Multiome | 967 | |
| chr19:1,285,317–1,286,108 | 193.4 kb | Distal (>10kb) Multiome | 277 | |
| chr19:1,354,303–1,355,411 | 124.3 kb | Distal (>10kb) Multiome | 688 | |
| chr19:1,383,122–1,384,333 | 95.5 kb | Distal (>10kb) Multiome | 1228 | |
| chr19:1,401,101–1,401,839 | 77.7 kb | Distal (>10kb) Multiome | 540 | |
| chr19:1,406,589–1,408,783 | 71.8 kb | Distal (>10kb) Multiome | 911 | |
| chr19:1,415,008–1,415,721 | 63.7 kb | Distal (>10kb) Multiome | 597 | |
| chr19:1,437,988–1,438,998 | 40.8 kb | Distal (>10kb) Multiome | 1076 | |
| chr19:1,444,785–1,447,383 | 34.0 kb | Distal (>10kb) Multiome | 797 | |
| chr19:1,455,191–1,456,421 | 23.4 kb | Distal (>10kb) Multiome | 491 | |
| chr19:1,468,941–1,469,903 | 9.9 kb | Proximal (<10kb) Multiome | 242 | |
| chr19:1,470,088–1,471,597 | 8.4 kb | Proximal (<10kb) Multiome | 544 | |
| chr19:1,478,772–1,479,848 | 137 bp | At TSS Multiome | 857 | |
| chr19:1,489,566–1,491,829 | 11.7 kb | Distal (>10kb) Multiome | 836 | |
| chr19:1,507,230–1,508,699 | 28.9 kb | Distal (>10kb) Multiome | 596 | |
| chr19:1,512,960–1,513,686 | 34.0 kb | Distal (>10kb) Multiome | 541 | |
| chr19:1,523,798–1,524,624 | 44.9 kb | Distal (>10kb) Multiome | 263 | |
| chr19:1,566,277–1,568,916 | 87.5 kb | Distal (>10kb) Multiome | 748 | |
| chr19:1,592,085–1,593,444 | 113.6 kb | Distal (>10kb) Multiome | 883 | |
| chr19:1,604,859–1,606,501 | 126.5 kb | Distal (>10kb) Multiome | 902 | |
| chr19:1,651,519–1,653,514 | 173.8 kb | Distal (>10kb) Multiome | 909 | |
| chr19:1,725,307–1,726,101 | 246.6 kb | Distal (>10kb) Multiome | 455 |
Genomic view of the C19orf25 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.