Bleomycin hydrolase (BMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution; however, the only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The protein contains the signature active site residues of the cysteine protease papain superfamily. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for BLMH. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = BLMH upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of BLMH, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr17:29,612,906–29,613,942 | 678.5 kb | Distal (>10kb) Multiome HiCAR | 581 | |
| chr17:29,614,997–29,616,357 | 676.4 kb | Distal (>10kb) Multiome HiCAR | 389 | |
| chr17:29,616,988–29,618,466 | 673.9 kb | Distal (>10kb) Multiome HiCAR | 615 | |
| chr17:30,104,595–30,105,065 | 187.2 kb | Distal (>10kb) Multiome | 585 | |
| chr17:30,116,470–30,117,077 | 175.2 kb | Distal (>10kb) Multiome | 800 | |
| chr17:30,291,298–30,291,449 | 494 bp | At TSS | 345 | |
| chr17:30,291,554–30,292,527 | 70 bp | At TSS Multiome | 724 | |
| chr17:30,340,166–30,340,693 | 48.4 kb | Distal (>10kb) Multiome | 394 | |
| chr17:30,378,665–30,379,625 | 86.8 kb | Distal (>10kb) Multiome | 693 | |
| chr17:30,477,182–30,477,726 | 185.3 kb | Distal (>10kb) Multiome | 903 |
Genomic view of the BLMH locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.