AZI2, or NAP1, contributes to the activation of NFKB (see MIM 164011)-dependent gene expression by activating IKK-related kinases, such as NAK (TBK1; MIM 604834) (Fujita et al., 2003 [PubMed 14560022]).[supplied by OMIM, Mar 2008]
Transcription factors with Perturb-seq knockdown data for AZI2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = AZI2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of AZI2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:27,680,718–27,681,875 | 667.8 kb | Distal (>10kb) Multiome HiCAR | 532 | |
| chr3:27,711,660–27,716,254 | 634.9 kb | Distal (>10kb) Multiome HiCAR | 783 | |
| chr3:27,720,744–27,724,896 | 626.3 kb | Distal (>10kb) Multiome HiCAR | 708 | |
| chr3:27,727,875–27,731,045 | 618.6 kb | Distal (>10kb) Multiome HiCAR | 446 | |
| chr3:28,240,444–28,242,372 | 107.4 kb | Distal (>10kb) Multiome | 1004 | |
| chr3:28,348,014–28,349,873 | 1 bp | At TSS Multiome | 1118 | |
| chr3:28,574,837–28,577,346 | 226.4 kb | Distal (>10kb) Multiome | 422 |
Genomic view of the AZI2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.