Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM, Mar 2008]
Transcription factors with Perturb-seq knockdown data for ATG12. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ATG12 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ATG12, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr5:115,543,835–115,545,478 | 297.0 kb | Distal (>10kb) Multiome | 943 | |
| chr5:115,601,724–115,602,813 | 239.7 kb | Distal (>10kb) Multiome | 542 | |
| chr5:115,625,502–115,626,687 | 215.7 kb | Distal (>10kb) Multiome | 976 | |
| chr5:115,785,567–115,786,155 | 56.0 kb | Distal (>10kb) Multiome | 90 | |
| chr5:115,815,539–115,817,207 | 25.2 kb | Distal (>10kb) Multiome | 490 | |
| chr5:115,838,984–115,839,487 | 2.1 kb | Proximal (<10kb) | 135 | |
| chr5:115,840,887–115,842,729 | 50 bp | At TSS Multiome | 1077 | |
| chr5:116,084,169–116,085,557 | 243.3 kb | Distal (>10kb) Multiome | 922 |
Genomic view of the ATG12 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.