Transcription factors with Perturb-seq knockdown data for ASH1L-AS1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ASH1L-AS1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ASH1L-AS1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:155,261,679–155,262,788 | 299.7 kb | Distal (>10kb) Multiome | 921 | |
| chr1:155,272,928–155,274,098 | 288.6 kb | Distal (>10kb) Multiome | 768 | |
| chr1:155,276,933–155,278,884 | 284.8 kb | Distal (>10kb) Multiome | 749 | |
| chr1:155,308,291–155,309,348 | 253.3 kb | Distal (>10kb) Multiome | 934 | |
| chr1:155,320,564–155,321,470 | 241.2 kb | Distal (>10kb) Multiome HiCAR | 341 | |
| chr1:155,322,680–155,325,643 | 237.7 kb | Distal (>10kb) Multiome HiCAR | 979 | |
| chr1:155,562,014–155,563,482 | 843 bp | At TSS Multiome | 909 | |
| chr1:155,609,731–155,610,295 | 48.0 kb | Distal (>10kb) Multiome | 503 | |
| chr1:155,688,087–155,689,328 | 126.8 kb | Distal (>10kb) Multiome | 838 | |
| chr1:155,745,339–155,745,870 | 183.6 kb | Distal (>10kb) Multiome | 505 | |
| chr1:155,856,932–155,857,529 | 295.2 kb | Distal (>10kb) Multiome | 639 | |
| chr1:155,859,095–155,860,745 | 298.5 kb | Distal (>10kb) Multiome | 632 |
Genomic view of the ASH1L-AS1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.