This gene encodes a member of the rhoGAP family of proteins which play a role in the regulation of small GTP-binding proteins belonging to the RAS superfamily. The protein encoded by the orthologous gene in rat is localized to the Golgi complex and can redistribute to microtubules. The rat protein stimulates the activity of some Rho GTPases in vitro. Genomic deletions of this gene and a neighboring gene have been found in patients with nephrogenic diabetes insipidus. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
Transcription factors with Perturb-seq knockdown data for ARHGAP4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ARHGAP4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ARHGAP4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chrX:153,687,144–153,688,247 | 239.1 kb | Distal (>10kb) Multiome | 201 | |
| chrX:153,723,829–153,725,285 | 202.2 kb | Distal (>10kb) Multiome | 620 | |
| chrX:153,726,395–153,727,168 | 199.9 kb | Distal (>10kb) Multiome | 529 | |
| chrX:153,793,808–153,795,045 | 132.4 kb | Distal (>10kb) Multiome | 733 | |
| chrX:153,875,415–153,875,856 | 51.2 kb | Distal (>10kb) Multiome | 847 | |
| chrX:153,878,887–153,879,684 | 47.7 kb | Distal (>10kb) Multiome | 235 | |
| chrX:153,926,011–153,927,020 | at TSS | At TSS | 493 | |
| chrX:153,927,955–153,928,728 | 1.6 kb | Proximal (<10kb) Multiome | 711 | |
| chrX:153,934,763–153,935,658 | 8.3 kb | Proximal (<10kb) Multiome | 446 | |
| chrX:153,970,913–153,973,065 | 45.6 kb | Distal (>10kb) Multiome | 857 | |
| chrX:154,097,040–154,098,161 | 171.0 kb | Distal (>10kb) Multiome | 537 | |
| chrX:154,136,648–154,137,146 | 210.0 kb | Distal (>10kb) Multiome | 391 |
Genomic view of the ARHGAP4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.