ARHGAPs, such as ARHGAP25, encode negative regulators of Rho GTPases (see ARHA; MIM 165390), which are implicated in actin remodeling, cell polarity, and cell migration (Katoh and Katoh, 2004 [PubMed 15254788]).[supplied by OMIM, Mar 2008]
Transcription factors with Perturb-seq knockdown data for ARHGAP25. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ARHGAP25 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ARHGAP25, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:68,466,807–68,468,080 | 212.1 kb | Distal (>10kb) Multiome | 955 | |
| chr2:68,642,929–68,644,096 | 36.1 kb | Distal (>10kb) Multiome | 454 | |
| chr2:68,679,240–68,679,770 | at TSS | At TSS | 188 | |
| chr2:68,943,373–68,944,527 | 264.3 kb | Distal (>10kb) Multiome | 424 |
Genomic view of the ARHGAP25 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.