This gene encodes a member of the GTPase activating protein family which activates a GTPase belonging to the RAS superfamily of small GTP-binding proteins. The encoded protein is insulin-responsive, is dependent on the kinase Akt and requires the Akt-dependent 14-3-3 binding protein which binds sequentially to two serine residues. The result of these interactions is regulation of cell motility. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
Transcription factors with Perturb-seq knockdown data for ARHGAP22. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ARHGAP22 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ARHGAP22, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr10:48,306,102–48,307,484 | 349.7 kb | Distal (>10kb) Multiome | 956 | |
| chr10:48,523,248–48,524,705 | 132.8 kb | Distal (>10kb) Multiome | 466 | |
| chr10:48,537,011–48,537,486 | 119.1 kb | Distal (>10kb) Multiome | 35 | |
| chr10:48,594,807–48,595,303 | 9.8 kb | Proximal (<10kb) | 66 | |
| chr10:48,605,051–48,605,410 | at TSS | At TSS | 142 | |
| chr10:48,608,212–48,608,651 | 3.1 kb | Proximal (<10kb) | 112 | |
| chr10:48,655,858–48,656,904 | 94 bp | At TSS Multiome | 603 | |
| chr10:48,658,341–48,658,750 | 2.1 kb | Proximal (<10kb) | 172 | |
| chr10:48,671,247–48,672,224 | 15.4 kb | Distal (>10kb) Multiome | 222 |
Genomic view of the ARHGAP22 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.