The protein encoded by this gene is a GTPase-activating protein (GAP) that associates with the Golgi apparatus and regulates the early secretory pathway of proteins. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1 (ARF1)-bound GTP, which is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is a prerequisite for the fusion of these vesicles with target compartments. The activity of this protein is sensitive to phospholipids. Multiple transcript variants encoding different isoforms have been found for this gene. This gene was originally known as ARFGAP1, but that is now the name of a related but different gene. [provided by RefSeq, Nov 2008]
Transcription factors with Perturb-seq knockdown data for ARFGAP3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ARFGAP3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ARFGAP3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr22:42,581,750–42,582,381 | 275.1 kb | Distal (>10kb) Multiome | 290 | |
| chr22:42,614,291–42,616,423 | 241.9 kb | Distal (>10kb) Multiome | 1280 | |
| chr22:42,648,972–42,649,906 | 207.8 kb | Distal (>10kb) Multiome | 625 | |
| chr22:42,662,035–42,662,743 | 195.0 kb | Distal (>10kb) Multiome | 468 | |
| chr22:42,720,289–42,721,050 | 136.4 kb | Distal (>10kb) Multiome | 297 | |
| chr22:42,800,630–42,801,305 | 56.2 kb | Distal (>10kb) Multiome | 559 | |
| chr22:42,856,601–42,857,716 | 32 bp | At TSS Multiome | 938 | |
| chr22:42,964,987–42,965,486 | 107.9 kb | Distal (>10kb) Multiome | 332 | |
| chr22:43,014,433–43,015,934 | 158.0 kb | Distal (>10kb) Multiome | 845 | |
| chr22:43,089,058–43,089,753 | 232.1 kb | Distal (>10kb) Multiome | 726 | |
| chr22:43,110,485–43,111,495 | 253.5 kb | Distal (>10kb) Multiome | 485 | |
| chr22:43,142,895–43,143,571 | 286.1 kb | Distal (>10kb) Multiome | 810 | |
| chr22:43,151,300–43,151,927 | 294.3 kb | Distal (>10kb) Multiome | 340 |
Genomic view of the ARFGAP3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.