This gene encodes a multi-pass transmembrane protein that belongs to the anoctamin family. This protein is an essential component for the calcium-dependent exposure of phosphatidylserine on the cell surface. The scrambling of phospholipid occurs in various biological systems, such as when blood platelets are activated, they expose phosphatidylserine to trigger the clotting system. Mutations in this gene are associated with Scott syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]
Transcription factors with Perturb-seq knockdown data for ANO6. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ANO6 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ANO6, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:44,948,600–44,949,248 | 267.2 kb | Distal (>10kb) Multiome | 709 | |
| chr12:45,049,985–45,051,809 | 165.3 kb | Distal (>10kb) Multiome | 372 | |
| chr12:45,191,976–45,193,002 | 23.5 kb | Distal (>10kb) Multiome HiCAR | 402 | |
| chr12:45,215,366–45,217,040 | 100 bp | At TSS Multiome | 944 | |
| chr12:45,232,686–45,233,194 | 16.8 kb | Distal (>10kb) Multiome | 341 | |
| chr12:45,268,666–45,269,446 | 53.0 kb | Distal (>10kb) Multiome HiCAR | 364 |
Genomic view of the ANO6 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.