Transcription factors with Perturb-seq knockdown data for ANKHD1-DT. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ANKHD1-DT upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ANKHD1-DT, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr5:140,107,138–140,108,849 | 293.6 kb | Distal (>10kb) Multiome | 993 | |
| chr5:140,112,921–140,114,941 | 287.4 kb | Distal (>10kb) Multiome | 811 | |
| chr5:140,145,842–140,146,580 | 255.3 kb | Distal (>10kb) Multiome | 366 | |
| chr5:140,157,178–140,157,756 | 244.1 kb | Distal (>10kb) Multiome | 504 | |
| chr5:140,174,682–140,176,221 | 226.4 kb | Distal (>10kb) Multiome | 959 | |
| chr5:140,302,631–140,303,538 | 98.3 kb | Distal (>10kb) Multiome | 552 | |
| chr5:140,345,869–140,347,147 | 54.8 kb | Distal (>10kb) Multiome | 671 | |
| chr5:140,363,055–140,363,697 | 38.1 kb | Distal (>10kb) Multiome | 542 | |
| chr5:140,372,779–140,373,538 | 28.4 kb | Distal (>10kb) Multiome | 302 | |
| chr5:140,400,395–140,402,925 | 95 bp | At TSS Multiome | 1129 | |
| chr5:140,546,917–140,548,500 | 146.2 kb | Distal (>10kb) Multiome | 784 | |
| chr5:140,556,971–140,557,959 | 156.0 kb | Distal (>10kb) Multiome | 886 | |
| chr5:140,564,055–140,565,123 | 163.1 kb | Distal (>10kb) Multiome | 827 | |
| chr5:140,639,043–140,640,146 | 238.0 kb | Distal (>10kb) Multiome | 898 | |
| chr5:140,647,175–140,648,159 | 246.2 kb | Distal (>10kb) Multiome | 863 | |
| chr5:140,664,368–140,665,161 | 263.3 kb | Distal (>10kb) Multiome | 856 | |
| chr5:140,673,200–140,673,759 | 272.1 kb | Distal (>10kb) Multiome | 144 | |
| chr5:140,691,053–140,691,933 | 290.0 kb | Distal (>10kb) Multiome | 924 | |
| chr5:140,699,981–140,700,742 | 298.9 kb | Distal (>10kb) Multiome | 607 |
Genomic view of the ANKHD1-DT locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.