The protein encoded by this gene is a type I transmembrane protein. It is thought to modulate bone morphogenetic protein (BMP) receptor function by serving as an accessory or coreceptor, and thus facilitates or hinders BMP binding. It is known that the mouse AMN gene is expressed in the extraembryonic visceral endoderm layer during gastrulation, but it is found to be mutated in amnionless mouse. The encoded protein has sequence similarity to short gastrulation (Sog) and procollagen IIA proteins in Drosophila. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for AMN. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = AMN upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of AMN, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr14:102,776,966–102,778,132 | 151.4 kb | Distal (>10kb) Multiome HiCAR | 446 | |
| chr14:102,927,667–102,929,100 | 288 bp | At TSS Multiome | 328 | |
| chr14:102,929,570–102,930,980 | 1.5 kb | Proximal (<10kb) Multiome | 178 | |
| chr14:102,954,209–102,955,338 | 26.1 kb | Distal (>10kb) Multiome | 143 | |
| chr14:103,056,337–103,058,497 | 128.9 kb | Distal (>10kb) Multiome | 644 | |
| chr14:103,075,052–103,076,162 | 146.9 kb | Distal (>10kb) Multiome | 656 | |
| chr14:103,091,357–103,091,855 | 162.8 kb | Distal (>10kb) Multiome | 384 | |
| chr14:103,104,655–103,105,370 | 176.3 kb | Distal (>10kb) Multiome | 618 | |
| chr14:103,122,605–103,123,921 | 194.2 kb | Distal (>10kb) Multiome | 378 | |
| chr14:103,126,688–103,127,597 | 198.3 kb | Distal (>10kb) Multiome | 313 | |
| chr14:103,188,912–103,189,569 | 260.4 kb | Distal (>10kb) Multiome | 138 | |
| chr14:103,207,129–103,207,830 | 278.6 kb | Distal (>10kb) Multiome | 200 |
Genomic view of the AMN locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.