ALOX5
arachidonate 5-lipoxygenase | 5-LOX

This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Biological processes 91 terms
arachidonate 12(S)-lipoxygenase activity (GO:0004052)arachidonate 5-lipoxygenase activity (GO:0004051)arachidonate 5-lipoxygenase activity (GO:0004051)arachidonate 5-lipoxygenase activity (GO:0004051)arachidonate 5-lipoxygenase activity (GO:0004051)arachidonate 8(S)-lipoxygenase activity (GO:0036403)arachidonate metabolic process (GO:0019369)cytoplasm (GO:0005737)cytosol (GO:0005829)cytosol (GO:0005829)cytosol (GO:0005829)cytosol (GO:0005829)dendritic cell migration (GO:0036336)dendritic cell migration (GO:0036336)extracellular region (GO:0005576)extracellular region (GO:0005576)ficolin-1-rich granule lumen (GO:1904813)glucose homeostasis (GO:0042593)glucose homeostasis (GO:0042593)humoral immune response (GO:0006959)humoral immune response (GO:0006959)icosanoid metabolic process (GO:0006690)iron ion binding (GO:0005506)iron ion binding (GO:0005506)leukocyte chemotaxis involved in inflammatory response (GO:0002232)leukocyte migration involved in inflammatory response (GO:0002523)leukocyte migration involved in inflammatory response (GO:0002523)leukotriene A4 biosynthetic process (GO:1901753)leukotriene A4 biosynthetic process (GO:1901753)leukotriene A4 biosynthetic process (GO:1901753)leukotriene biosynthetic process (GO:0019370)leukotriene metabolic process (GO:0006691)lipid oxidation (GO:0034440)lipid oxidation (GO:0034440)lipoxin biosynthetic process (GO:2001301)lipoxin biosynthetic process (GO:2001301)lipoxygenase pathway (GO:0019372)lipoxygenase pathway (GO:0019372)long-chain fatty acid biosynthetic process (GO:0042759)long-chain fatty acid metabolic process (GO:0001676)metal ion binding (GO:0046872)negative regulation of angiogenesis (GO:0016525)negative regulation of angiogenesis (GO:0016525)negative regulation of endothelial cell proliferation (GO:0001937)negative regulation of endothelial cell proliferation (GO:0001937)negative regulation of inflammatory response (GO:0050728)negative regulation of response to endoplasmic reticulum stress (GO:1903573)negative regulation of response to endoplasmic reticulum stress (GO:1903573)negative regulation of sprouting angiogenesis (GO:1903671)negative regulation of sprouting angiogenesis (GO:1903671)negative regulation of vascular wound healing (GO:0061044)negative regulation of vascular wound healing (GO:0061044)negative regulation of wound healing (GO:0061045)negative regulation of wound healing (GO:0061045)nuclear envelope (GO:0005635)nuclear envelope (GO:0005635)nuclear envelope (GO:0005635)nuclear envelope (GO:0005635)nuclear envelope lumen (GO:0005641)nuclear envelope lumen (GO:0005641)nuclear matrix (GO:0016363)nuclear matrix (GO:0016363)nuclear membrane (GO:0031965)nuclear membrane (GO:0031965)nucleoplasm (GO:0005654)nucleus (GO:0005634)oxidoreductase activity (GO:0016491)oxidoreductase activity, acting on single donors with incorporation of molecular oxygen (GO:0016701)oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen (GO:0016702)perinuclear region of cytoplasm (GO:0048471)perinuclear region of cytoplasm (GO:0048471)positive regulation of bone mineralization (GO:0030501)positive regulation of bone mineralization (GO:0030501)positive regulation of leukocyte adhesion to arterial endothelial cell (GO:1904999)positive regulation of leukocyte adhesion to arterial endothelial cell (GO:1904999)protein binding (GO:0005515)regulation of cellular response to oxidative stress (GO:1900407)regulation of cellular response to oxidative stress (GO:1900407)regulation of cytokine production involved in inflammatory response (GO:1900015)regulation of cytokine production involved in inflammatory response (GO:1900015)regulation of fat cell differentiation (GO:0045598)regulation of fat cell differentiation (GO:0045598)regulation of inflammatory response (GO:0050727)regulation of inflammatory response (GO:0050727)regulation of inflammatory response to wounding (GO:0106014)regulation of inflammatory response to wounding (GO:0106014)regulation of insulin secretion (GO:0050796)regulation of insulin secretion (GO:0050796)regulation of reactive oxygen species biosynthetic process (GO:1903426)response to stress (GO:0006950)secretory granule lumen (GO:0034774)
Expression (TPM)
ALOX5 — as a Regulated Gene

TFs regulating ALOX5 0 TFs

Transcription factors with Perturb-seq knockdown data for ALOX5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ALOX5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

Data: Effect:
TF Mean coef Binding Outlier TF→Gene link

Elements linked to ALOX5

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ALOX5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Accessibility Element Dist. to TSS Link type TFs
chr10:45,373,156–45,374,935 at TSS At TSS 443

Genome Browser

Genomic view of the ALOX5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.

chr10:45,363,156 – 45,384,935
Proximal 1 kb Distal 10 kb Multiome HiCAR ATAC-seq RNA-seq