Enables mRNA N6-methyladenosine dioxygenase activity and molecular condensate scaffold activity. Involved in membraneless organelle assembly; post-transcriptional regulation of gene expression; and response to hypoxia. Acts upstream of or within regulation of mRNA export from nucleus and regulation of mRNA processing. Located in Golgi apparatus; cytosol; and nuclear speck. Is active in paraspeckles. [provided by Alliance of Genome Resources, Jul 2025]
Transcription factors with Perturb-seq knockdown data for ALKBH5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ALKBH5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ALKBH5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr17:17,921,363–17,921,988 | 262.1 kb | Distal (>10kb) Multiome | 341 | |
| chr17:17,943,271–17,943,954 | 240.3 kb | Distal (>10kb) Multiome | 143 | |
| chr17:17,971,984–17,973,164 | 211.2 kb | Distal (>10kb) Multiome | 716 | |
| chr17:18,035,659–18,036,594 | 147.8 kb | Distal (>10kb) Multiome | 186 | |
| chr17:18,038,656–18,040,279 | 144.5 kb | Distal (>10kb) Multiome | 839 | |
| chr17:18,153,365–18,154,352 | 29.9 kb | Distal (>10kb) Multiome | 244 | |
| chr17:18,157,790–18,158,725 | 25.6 kb | Distal (>10kb) Multiome | 292 | |
| chr17:18,182,585–18,185,065 | 319 bp | At TSS Multiome | 1051 | |
| chr17:18,224,148–18,226,173 | 40.9 kb | Distal (>10kb) Multiome | 680 | |
| chr17:18,246,777–18,247,912 | 63.5 kb | Distal (>10kb) Multiome | 314 | |
| chr17:18,257,865–18,259,083 | 74.8 kb | Distal (>10kb) Multiome | 684 | |
| chr17:18,260,145–18,261,582 | 76.8 kb | Distal (>10kb) Multiome | 778 | |
| chr17:18,314,513–18,315,715 | 131.3 kb | Distal (>10kb) Multiome | 865 | |
| chr17:18,363,090–18,363,830 | 179.7 kb | Distal (>10kb) Multiome | 760 |
Genomic view of the ALKBH5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.