This gene encodes a member of the glycosyltransferase 22 family. The encoded protein catalyzes the addition of the eighth mannose residue in an alpha-1,6 linkage onto the dolichol-PP-oligosaccharide precursor (dolichol-PP-Man(7)GlcNAc(2)) required for protein glycosylation. Mutations in this gene have been associated with congenital disorder of glycosylation type Ig (CDG-Ig)characterized by abnormal N-glycosylation. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for ALG12. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ALG12 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ALG12, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr22:49,852,925–49,854,755 | 64.7 kb | Distal (>10kb) Multiome | 864 | |
| chr22:49,917,887–49,919,285 | 30 bp | At TSS Multiome | 736 | |
| chr22:49,934,630–49,935,498 | 17.0 kb | Distal (>10kb) Multiome | 298 | |
| chr22:49,935,964–49,936,517 | 17.6 kb | Distal (>10kb) Multiome | 637 | |
| chr22:49,959,889–49,961,612 | 42.0 kb | Distal (>10kb) Multiome | 843 | |
| chr22:49,961,773–49,962,968 | 44.0 kb | Distal (>10kb) Multiome | 708 | |
| chr22:49,999,173–50,000,437 | 81.2 kb | Distal (>10kb) Multiome | 237 | |
| chr22:50,030,288–50,030,739 | 112.0 kb | Distal (>10kb) Multiome | 433 | |
| chr22:50,169,918–50,170,446 | 251.8 kb | Distal (>10kb) Multiome | 245 | |
| chr22:50,184,288–50,186,776 | 267.4 kb | Distal (>10kb) Multiome | 542 | |
| chr22:50,199,706–50,201,679 | 282.5 kb | Distal (>10kb) Multiome | 612 |
Genomic view of the ALG12 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.