Transcription factors with Perturb-seq knockdown data for AK3P5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = AK3P5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of AK3P5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr10:32,938,653–32,939,083 | 5.4 kb | Proximal (<10kb) | 196 | |
| chr10:32,944,155–32,944,554 | at TSS | At TSS | 447 | |
| chr10:32,948,457–32,949,002 | 4.0 kb | Proximal (<10kb) | 314 | |
| chr10:32,950,586–32,951,108 | 6.1 kb | Proximal (<10kb) | 209 | |
| chr10:32,954,253–32,954,452 | 9.8 kb | Proximal (<10kb) | 81 |
Genomic view of the AK3P5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.