Transcription factors with Perturb-seq knockdown data for AHI1-DT. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = AHI1-DT upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of AHI1-DT, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr6:135,497,226–135,498,795 | at TSS | At TSS | 1084 | |
| chr6:135,502,596–135,502,930 | 4.8 kb | Proximal (<10kb) | 7 | |
| chr6:135,503,753–135,504,314 | 6.0 kb | Proximal (<10kb) | 125 | |
| chr6:135,506,402–135,506,539 | 8.6 kb | Proximal (<10kb) | 91 |
Genomic view of the AHI1-DT locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.