The ADHFE1 gene encodes hydroxyacid-oxoacid transhydrogenase (EC 1.1.99.24), which is responsible for the oxidation of 4-hydroxybutyrate in mammalian tissues (Kardon et al., 2006 [PubMed 16616524]).[supplied by OMIM, Mar 2008]
Transcription factors with Perturb-seq knockdown data for ADHFE1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ADHFE1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ADHFE1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr8:66,176,683–66,178,388 | 255.1 kb | Distal (>10kb) Multiome | 818 | |
| chr8:66,228,525–66,229,416 | 203.6 kb | Distal (>10kb) Multiome | 92 | |
| chr8:66,348,361–66,349,034 | 83.9 kb | Distal (>10kb) Multiome | 176 | |
| chr8:66,428,113–66,430,401 | 3.7 kb | Proximal (<10kb) Multiome | 921 | |
| chr8:66,431,895–66,432,840 | 2 bp | At TSS Multiome | 609 | |
| chr8:66,436,175–66,436,416 | 3.7 kb | Proximal (<10kb) | 21 | |
| chr8:66,438,980–66,439,800 | 6.5 kb | Proximal (<10kb) | 484 | |
| chr8:66,612,352–66,614,219 | 180.9 kb | Distal (>10kb) Multiome | 862 | |
| chr8:66,666,261–66,667,884 | 234.9 kb | Distal (>10kb) Multiome | 889 | |
| chr8:66,712,294–66,713,281 | 280.2 kb | Distal (>10kb) Multiome | 648 |
Genomic view of the ADHFE1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.